Murphy died Wednesday in New York of leukemia, according to his representative, Domenick Nati. He was perhaps best-known for his appearances on Chappelle's Show on Comedy Central.
He collaborated with writing his brother's starring films, Norbert and Vampire in Brooklyn. He voiced roles in animated TV series that include The Boondocks and Black Jesus.
Murphy's feature films include Our Family Wedding, King's Ransom and CB4.
He is credited with appearances to air later this year on the TV drama series Power.
Dave Chappelle honoured late comedian and Chappelle's Show cast member Charlie Murphy while making a cameo at a John Mayer concert in Columbus, Ohio Wednesday.
"Today, I got some terrible news," Chappelle said. "My good friend, Charlie Murphy, passed away this morning, and everybody in comedy is heartbroken." The comedian and Mayer reminisced about Murphy's legendary "True Hollywood Stories" sketches on Chappelle's Show, with both noting they still use Murphy's classic line about Rick James, "He's a habitual line-stepper."
Chappelle then asked Mayer to perform a special rendition of "You're Gonna Live Forever in Me," which the comedian said reminded him of Murphy